roxadustat 15294  (Cayman Chemical)

Journal: Molecules

Article Title: Inhibition of VHL by VH298 Accelerates Pexophagy by Activation of HIF-1α in HeLa Cells

doi: 10.3390/molecules29020482

Figure Lengend Snippet: VH298 induces pexophagy via HIF-1α activation. ( A ) RPE/mRFP–EGFP–SKL cells pretreated with VH298 (50 µM) for 42 h were further incubated with or without cycloheximide (10 µg/mL) for 6 h. The cells were then imaged by using a fluorescence microscope. The percentage of puncta exhibiting EGFP(+)- and mRFP(+)-labeled autophagosomes or EGFP(−)- and mRFP(+)-labeled autolysosomes per cell was determined. The scale bar indicates 20 µm (data are presented as mean ± standard error of the mean; n = 100 and *** p < 0.001). ( B ) RPE/mRFP–EGFP–SKL cells were treated with VH298 (50 µM), VL285 (50 µM), and roxadustat (50 µM) for 48 h and then imaged by using a fluorescence microscope. Subsequently, the fluorescence intensities of EGFP and mRFP were captured and quantified. The scale bar indicates 20 µm (data are presented as mean ± standard error of the mean; n = 100 and *** p < 0.001). ( C ) RPE cells were treated with VH298 (50 µM) and roxadustat (50 µM) for 48 h, after which they were analyzed via Western blotting by using the indicated antibodies. ( D ) RPE/mRFP-EGFP-SKL cells were transiently transfected with scrambled siRNA (Sc), siRNA targeting HIF-1α for 48 h, and then treated with or without VH298 (50 µM) for 48 h. The cells were then imaged by using a fluorescence microscope. The percentage of puncta exhibiting EGFP(+)- and mRFP(+)-labeled autophagosomes or EGFP(−)- and mRFP(+)-labeled autolysosomes per cell was determined. The scale bar indicates 20 µm (data are presented as mean ± standard error of the mean; n = 100 and *** p < 0.001).

Article Snippet: Roxadustat (15294) was obtained from Cayman Chemical Co. (Ann Arbor, MI, USA).

Techniques: Activation Assay, Incubation, Fluorescence, Microscopy, Labeling, Western Blot, Transfection

Journal: Nutrients

Article Title: Acai Extract Transiently Upregulates Erythropoietin by Inducing a Renal Hypoxic Condition in Mice

doi: 10.3390/nu12020533

Figure Lengend Snippet: Acai extract upregulates both the plasma erythropoietin (EPO) concentration and kidney Epo expression. ( A ). The relative Epo transcript level in the kidney after the oral administration of acai extract (10 g/kg) dairy for four days. * P < 0.05 by t -test. ( B ) The plasma EPO concentration in mice 2–3 h after the oral administration of acai extract (10 g/kg), C3Glc (50 mg/kg), and ASP1517 (80 mg/kg). ( C ) Relative Epo transcript levels in kidney 2–3 h after the oral administration of acai extract (10 g/kg), C3Glc (50 mg/kg), and ASP1517 (80 mg/kg). ( D ) Relationship between the plasma EPO concentration and Epo transcription in kidney. Error bars indicate the standard deviation. * P < 0.05 by an ANOVA/Tukey’s test.

Article Snippet: ASP1517 (roxadustat, #15294) was purchased from Cayman Chemical (Ann Arbor, MI, USA).

Techniques: Clinical Proteomics, Concentration Assay, Expressing, Standard Deviation